Telephone: (303) 724-3167; Fax: (303) 724-3889
Email:
DNA.Core@UCHSC.edu
Web
Site: http://loki.uchsc.edu
The CU CANCER CENTER DNA SEQUENCING CORE
was established in the fall of 1995. It offers rapid sequencing of DNA to
Cancer Center members as well as to academic and industrial researchers in
Colorado. The Core started with two automated single-lane, four-color
fluorescent slab-gel automated sequencers (ABI 373A-XL and ABI 377-XL).
These have been replaced with two automated DNA sequencers; a 16-capillary (ABI
3100) and a 48-capillary (ABI 3730, the only in Colorado), which can provide
over 650 and 900 bp of usable DNA sequence, respectively. The Core also
offers DNA profiling for identification and authentication of cell lines.
In the near future, the Core will start to offer CAP-approved screening of
clinical samples for mutations in the exons of specific genes implicated in
cancer (e.g., VHL, p53, pTEN, FLT3).
Customers can supply the DNA as one of the
following: plasmid DNA, an E. coli transformant bearing plasmid DNA, DNA
fragments (restriction or PCR products), lambda phage DNA, cosmid DNA, PAC DNA,
or BAC DNA. A Beckman Biomek 2000 robot allows the Core to offer automated,
high-throughput template (plasmid) preparation for large-scale sequencing
projects and preparation of high density arrays on membrane filters of
different gene libraries.
The Core offers expert technical advice on
the best strategy for individual sequencing projects, troubleshooting of PCR
and DNA sequencing projects, and analysis of sequences with a suite of computer
programs. The results are provided, in confidence, as chromatograms and
computer disk files, with backup copies kept in-house. These services are
priced very competitively for both academic and industrial researchers.
These services are provided on the
condition that the Core_s customers acknowledge use of the CU-CANCER CENTER DNA
SEQUENCING CORE's services in their publications and provide the Core with
information of the grants that support the use of the Core. Please
contact the Core for specific details.